Hemophilia and von Willebrand's disease are the most common congenital bleeding disorders. Hemophilia refers to X-linked bleeding disorders in which there is a deficiency (activity level of 35 % or less) of either factor VIII (hemophilia A, classic hemophilia) or factor IX (hemophilia B, Christmas disease). In general, administration of anti-hemophilic factor is indicated for hemophilia when a bleeding episode arises (demand treatment) or when bleeding is anticipated or likely (prophylactic treatment).
Primary prophylactic therapy may be indicated for patients with severe hemophilia A or hemophilia B who have less than 1 % of normal factor (less than 0.01 IU/ml; National Hemophilia Foundation, 2001). Primary prophylactic therapy should be instituted early, prior to the onset of frequent bleeding, with the aim of keeping the trough factor or VIII or factor IX level above 1 % between doses (National Hemophilia Foundation, 2001). In some cases, continuous prophylactic therapy may be indicated in persons with hemophilia A or hemophilia B that is not severe (i.e., hemophiliacs with more than 1 % of normal factor levels) who have repeated episodes of spontaneous bleeding.
Short-term prophylactic treatment is given to patients before they undergo surgical procedures or engage in activities that carry a high risk of provoking a bleed. It may also be given to break the cycle of frequent bleeding into specific joints (target joints). Potential harm, including the risk of hepatitis B, hepatitis C and HIV infection, has now been minimized through viral inactivation of plasma-derived coagulation-factor concentrates and through the use of recombinant clotting factor concentrates and other non-plasma-derived hemostatic agents. Currently, cryoprecipitate does not undergo viral inactivation procedures.
Immune tolerance induction is designed to overcome the effects of anti-hemophilic factor or factor IX inhibitors in certain hemophiliac patients, thus restoring effectiveness of antihemophilic factor or factor IX therapy to resolve active bleeding in these patients. It consists of administration of very high doses of anti-hemophilic factor or factor IX over an extended period of time.
A number of different immune tolerance therapy regimens have been developed using replacement factor VIII or factor IX to produce long-term inhibitor suppression or eradication. These protocols utilize various doses of replacement factor VIII or factor IX with or without additional therapies.
There are a number of established protocols for immune tolerance induction; these regimens differ in dose and duration of therapy (Stachnik, 2003). The North American Immune Tolerance Registry was established in 1993 to assess the various protocols used for immune tolerance therapy in the United States and Canada (Stachnik, 2003; Kroner, 1999; DiMichele and Kroner, 1999). Based on a survey of 168 centers that provide medical care to hemophilia patients, many clinicians in North America (about 50 %) use 100 to 200 U/kg of replacement factor VIII daily for immune tolerance therapy. In general, the duration of treatment is shorter when using higher doses of factor VIII. For doses of factor VIII over 50 U/kg daily, the mean treatment time was between 6 and 7 months; however, with lower doses of factor VIII (less than 50 U/kg daily), the mean duration of treatment was nearly 19 months. Highest success rates were found among patients who had a history of low maximum inhibitor titers (less than 50 Bethesda Units).
Immune tolerance induction is not successful in all patients -- approximately 20 % to 30 % fail to respond (Stachnik, 2003). Although the duration of treatment varies among the immune tolerance protocols, most clinicians would agree that a patient who has not responded as expected after 12 to 24 months of treatment is unlikely to respond with further treatment. Re-induction tolerance, although occasionally successful, generally is not effective (Stachnik, 2003). For these patients, bleeding episodes can usually be controlled using bypassing agents, including factor IX complex (prothrombin complex concentrates or PCCs), recombinant factor VIIa (rFVIIa, NovoSeven RT), or activated anti-inhibitor coagulant complexes (AICCs or aPCCs).
In view of the increasing safety of clotting-factor concentrates, long-term prophylactic therapy in the form of factor VIII infusion at least 3 times a week or factor IX infusion at least twice a week to prevent hemarthrosis in severely affected patients is gaining acceptance, especially in the treatment of infants and children. It has been shown that increasing in-vivo clotting-factor levels to more than 1 % activity (usually accomplished by giving 25 to 40 U/kg of factor VIII 3 times a week or 25 to 40 U/kg of factor IX twice a week) is sufficient to prevent most spontaneous joint bleeds and preserve joint function.
von Willebrand's disease is typically mild and it generally exhibits an autosomal dominant pattern of inheritance. Anti-hemophilic factor/von Willebrand factor complex (Humate-P) is indicated for use in adult patients for treatment and prevention of bleeding in hemophilia A and in adult and pediatric patients for treatment of spontaneous and trauma-induced bleeding episodes in severe von Willebrand disease and in mild and moderate von Willebrand disease where use of desmopressin is known or suspected to be inadequate. In April 2007, the U.S. Food and Drug Administration (FDA) approved Humate-P for the prevention of excessive bleeding during and after surgery in certain patients with mild-to-moderate and severe von Willebrand disease.
Recombinant antihemophilic factor -- plasma/albumin free method (rAHF-PFM) (Advate, Baxter Healthcare, Westlake Village, CA) is produced using a protein-free manufacturing process. Based on clinical studies submitted to the FDA comparing rAHF-PFM to a standard recombinant antihemophilic factor (rAHF) (Recombinate, Baxter Healthcare), the FDA has concluded that rAHF-PFM is therapeutically equivalent to rAHF. Human or animal plasma proteins or albumin are used in the cell-culture process of rAHF, with the theoretical risk of transmission viruses, prions or other pathogens (Schesinger and Ragni, 2002; Adis, 2003). The Medical and Scientific Advisory Council (MASAC) of the National Hemophilia Foundation has recommended that "all efforts should be made to remove human albumin from recombinant factor VIII products" and that "increased efforts should be made to eliminate human and bovine proteins from the manufacturing process of recombinant products." However, there are no documented episodes of transmission of HIV, West Nile Virus, Creutzfeld-Jacob Disease or other such viral pathogens from rAHF products currently on the market.
Case reports have been published on the successful use of rFVIIa in patients with Glanzmann's thrombasthenia, which is an inherited hemorrhagic disorder characterized by a severe reduction in, or absence of, platelet aggregation in response to multiple physiologic agonists due to qualitative or quantitative abnormalities of platelet glycoprotein IIb-IIIa. The European Medicine’s Agency (EMEA) has approved rFVIIa for use in persons with Glanzmann’s thrombasthenia with antibodies to glycoprotein IIb-IIIa and/or human leukocyte antigen (HLA), and with past or present refractoriness to platelet transfusions. A scientific discussion prepared by the EMEA (2005) states that the clinical experience with the treatment of patients with Glanzmann’s thrombasthenia with rFVIIa is based on data from a clinical trial (n = 4), an International Registry on Recombinant Factor VIIa and Congenital Platelet Disorders (n = 59), and 10 published case reports. Most of the reported clinical experience with the use of rFVIIa in persons with Glanzmann’s thrombasthenia is in patients without anti-glycoprotein antibodies and platelet refractoriness. The EMEA analyzed the efficacy data for persons with anti-glycoprotein IIb/IIIa and/or anti-HLA antibodies and platelet refractoriness. Seventeen patients with from the International Registry and 2 patients from independently published case reports were identified, with a total of 40 evaluable bleeding episodes. The EMEA scientific discussion reports that rFVIIa was effective for bleeding prophylaxis in 18 (95 %) of 19 evaluable (minor plus major) surgical procedures. The EMEA states that rFVIIa was effective in stopping 28/40 (70 %) of the evaluable bleeding episodes, which is similar as the efficacy of rFVIIa in bleeding episodes reported in Glanzmann’s thrombasthenia patients generally. The EMEA scientific discussion states that 3 recurrent bleedings (3/40, 8 %) could be successfully treated with additional doses of rFVIIa (but in 1 case a concomitant platelet transfusion was given).
There is a lack of reliable evidence of the effectiveness of rFVIIa for non-hemophilic indications. Levi and colleagues (2005) performed a systematic review on the safety and effectiveness of rFVIIa in patients with or without coagulation disorders. They concluded that more randomized controlled clinical trials are needed to evaluate the safety and effectiveness of rFVIIa for patients without a pre-existent coagulation disorder and with severe bleeding. In the meantime, off-labeled use of rFVIIa may be considered in patients with life-threatening bleeding. This is in agreement with the observations of Lam et al (2005) who stated that until further prospective controlled data are available, it is recommended that conventional intervention for prevention and control of hemorrhage in non-hemophiliac patients should remain the standard of care. More recently, O’Connell and colleagues (2006) reported that the use of rFVIIa is associated with severe adcerse events (AEs) especially when it is used for off-labeled indications. They analyzed 431 incidents of AEs reported to the FDA during the first 5 years after the approval of NovoSeven. They noted that most reported thromboembolic AEs followed the use of the drug for off-labeled indications (n = 151) and occurred in the arterial and venous systems, often resulting in serious morbidity and mortality. The majority of complications occurred within 24 hours of taking the drug. These investigators concluded that randomized controlled studies are needed to ascertain the safety and effectiveness of rFVIIa in patients without hemophilia.
A number of studies have found that hemostatic therapy with rFVIIa may reduce growth of hematoma but does not improve survival or functional outcome after intra-cerebral hemorrhage. In a prospective, randomized, placebo-controlled, dose-escalation study, Narayan and colleagues (2008) evaluated the safety and preliminary effectiveness of rFVIIa to limit traumatic intra-cerebral hemorrhage (tICH) progression. Patients were enrolled if they had tICH lesions of at least 2 ml on a baseline computed tomographic scan obtained within 6 hours of injury. Recombinant factor VIIa or placebo was administered within 2.5 hours of the baseline computed tomographic scan but no later than 7 hours after injury. Computed tomographic scans were repeated at 24 and 72 hours. Five escalating dose tiers were evaluated (40, 80, 120, 160, and 200 microg/kg rFVIIa). Clinical evaluations and AEs were recorded until day 15. No significant differences were detected in mortality rate or number and type of AEs among treatment groups. Asymptomatic deep vein thrombosis, detected on routinely performed ultrasound at day 3, was observed more frequently in the combined rFVIIa treatment group (placebo, 3 %; rFVIIa, 8 %; not significant). A non-significant trend for rFVIIa dose-response to limit tICH volume increase was observed (placebo, 21.0 ml; rFVIIa, 10.1 ml). The authors concluded that in this first prospective study of rFVIIa in tICH, there appeared to be a trend toward less hematoma progression in rFVIIa-treated patients (80 to 200 microg/kg) compared with that seen in placebo-treated patients. The authors stated that the potential significance of this biological effect on clinical outcomes and the significance of the somewhat higher incidence of ultrasound-detected deep vein thromboses in the rFVIIa-treated group need to be examined in a larger prospective randomized clinical trial.
Mayer et al (2008) carried out a phase 3 trial to evaluate whether rFVIIa reduces growth of hematoma and improves survival and functional outcomes in patients with ICH. A total of 841 patients were randomly assigned to receive placebo (n = 268), 20 microg of rFVIIa per kilogram of body weight (n = 276), or 80 microg of rFVIIa per kilogram (n = 297) within 4 hours after the onset of stroke. The primary end point was poor outcome, defined as severe disability or death according to the modified Rankin scale 90 days after the stroke. Treatment with 80 microg of rFVIIa per kilogram resulted in a significant reduction in growth in volume of the hemorrhage. The mean estimated increase in volume of the ICH at 24 hours was 26 % in the placebo group, as compared with 18 % in the group receiving 20 microg of rFVIIa per kilogram (p = 0.09) and 11 % in the group receiving 80 microg (p < 0.001). The growth in volume of ICH was reduced by 2.6 ml (95 % confidence interval [CI]: -0.3 to 5.5; p = 0.08) in the group receiving 20 microg of rFVIIa per kilogram and by 3.8 ml (95 % CI: 0.9 to 6.7; p = 0.009) in the group receiving 80 microg, as compared with the placebo group. Despite this reduction in bleeding, there was no significant difference among the 3 groups in the proportion of patients with poor clinical outcome (24 % in the placebo group, 26 % in the group receiving 20 microg of rFVIIa per kilogram, and 29 % in the group receiving 80 microg). The overall frequency of thromboembolic serious AEs was similar in the 3 groups; however, arterial events were more frequent in the group receiving 80 microg of rFVIIa than in the placebo group (9 % versus 4 %, p = 0.04). The authors concluded that hemostatic therapy with rFVIIa reduced growth of the hematoma but did not improve survival or functional outcome after ICH. Whether this hemostatic effect can translate to clinical benefit in a subgroup of patients at high risk for active bleeding, either by treatment within an earlier time window or by demonstration of intra-hematomal contrast extravasation after CT angiography deserves further study.
A randomized controlled study found no effect of rFVIIa on a primary composite endpoint of failure to control 24-hr variceal bleeding, failure to control rebleeding or death in patients with advanced cirrhosis. In a randomized controlled study, Bosch and associates (2008) evaluated the safety and effectiveness of rFVIIa in patients with advanced cirrhosis and active variceal bleeding. At 31 hospitals in an emergency setting, 256 patients (Child-Pugh greater than 8; Child-Pugh B = 26 %, C = 74 %) were randomized equally to: placebo; 600 microg/kg rFVIIa (200 + 4x 100 microg/kg); or 300 microg/kg rFVIIa (200 + 100 microg/kg). Dosing was intravenous at 0, 2, 8, 14, and 20 hrs after endoscopy, in addition to standard vasoactive, prophylactic antibiotic, and endoscopic treatment. The primary composite endpoint consisted of failure to control 24-hr bleeding, or failure to prevent re-bleeding or death at day 5. Secondary endpoints included AEs and 42-day mortality. Baseline characteristics were comparable between groups. Administration of rFVIIa had no significant effect on the composite endpoint compared with placebo (p = 0.37). There was no significant difference in 5-day mortality between groups; however, 42-day mortality was significantly lower with 600 microg/kg rFVIIa compared with placebo (odds ratio 0.31, 95 % CI: 0.13 to 0.74), and bleeding-related deaths were reduced from 12 % (placebo) to 2 % (600 microg/kg). A marked heterogeneity in the failure rate in all treatment groups was observed across participating centers. Adverse events, including overall thromboembolic events, were comparable between groups. The authors concluded that treatment with rFVIIa had no significant effect on the primary composite endpoint compared with placebo. Thus, decision on the use of rFVIIa in acute variceal bleeding should be carefully considered, because results of this study do not support the routine use of rFVIIa in this setting.
A Cochrane systematic evidence review (Marti-Carvajal et al, 2007) concluded that they found no evidence that rFVIIa reduces the risk of death in patients with liver disease and upper gastrointestinal bleeding. "More randomised clinical trials having low risk of bias are necessary in order to determine the role of human recombinant factor VIIa in clinical practice."
An assessment by the Canadian Coordinating Office for Health Technology Assessment (Selin and Tejani, 2006) reported that rFVIIa is increasingly used to control bleeding in non-hemophilic patients during surgery, or during treatment for severe trauma or ICH. The assessment noted that there is potential for non-hemophilic use of rFVIIa, particularly if clinical and cost-effectiveness are established. The assessment concluded that adequately powered randomized controlled trials are needed to clarify the efficacy and safety of rFVIIa for non-bleeding disorder indications. The assessment noted that phase III trials in ICH and trauma are underway.
A randomized controlled clinical study found that propylactic use of rFVIIa did not decrease perioperative blood loss in patients with normal hemostasis undergoing surgical repair of traumatic pelvic-acetabular fracture. In a double-blind, randomized, placebo-controlled trial, Raobaikady and associates (2005) examined the clinical value of rFVIIa in patients undergoing reconstruction surgery for traumatic fracture of pelvis or pelvis and acetabulum (n = 48). Patients were randomized to receive an intravenous bolus injection of rFVIIa 90 microg/kg or placebo as add-on therapy at the time of the first skin incision. All patients also received intra-operative salvaged red blood cells (RBC). There was no significant difference in the total volume of peri-operative blood loss, the primary outcome variable, between the rFVIIa and placebo groups. In addition, there were no differences between the 2 groups in the total volume of blood components, including salvaged RBC transfused, number of patients requiring allogeneic blood components, total volume of fluids infused, total operating time, time taken after entry to the intensive care unit to reach normal body temperature and acid-base status, and time spent in hospital. No adverse events, in particular thromboembolic events, were reported in either group. The authors concluded that in patients with normal hemostasis undergoing repair surgery of traumatic pelvic-acetabular fracture, the prophylactic use of rFVIIa does not decrease the volume of peri-operative blood loss.
A review found that the evidence does not support the use of rFVIIa to prevent or control excessive bleeding after cardiac surgery. Hardy and co-workers (2009) noted that excessive bleeding is a common and morbid problem after cardiac surgery. There is no doubt a need for an effective and safe hemostatic agent in order to minimize transfusions and avoid surgical re-intervention for hemostasis. Recombinant activated factor VII is being used (off-label) increasingly after cardiac surgery to prevent or to control hemorrhage, but its efficacy and safety remain unclear. Several case reports, case series and registries would tend to support the use of rFVIIa to control excessive bleeding after cardiac operations. On the contrary, 2 randomized controlled trials have produced negative results whereas a 3rd has not been published yet. Adverse thrombotic events have been reported with increasing frequency. The authors concluded that at present, the generalized use of rFVIIa to prevent or to control excessive bleeding after cardiac surgery can not be recommended.
Analaysis of a large cohort of persons in placebo-controlled trials of rFVIIa found that treatment with high doses of rFVIIa on an off-label basis significantly increased the risk of arterial thromboembolic events. Levi and colleagues (2010) evaluated the rate of thromboembolic events in all published randomized, placebo-controlled trials of rFVIIa used on an off-label basis (i.e., non-hemophilia). These investigators analyzed data from 35 randomized clinical trials (26 studies involving patients and 9 studies involving healthy volunteers) to determine the frequency of thromboembolic events. The data were pooled with the use of random-effects models to calculate the odds ratios and 95 % CI. Among 4,468 subjects (4,119 patients and 349 healthy volunteers), 498 had thromboembolic events (11.1 %). Rates of arterial thromboembolic events among all 4,468 subjects were higher among those who received rFVIIa than among those who received placebo (5.5 % versus 3.2 %, p = 0.003). Rates of venous thromboembolic events were similar among subjects who received rFVIIa and those who received placebo (5.3 % versus 5.7 %). Among subjects who received rFVIIa, 2.9 % had coronary arterial thromboembolic events, as compared with 1.1 % of those who received placebo (p = 0.002). Rates of arterial thromboembolic events were higher among subjects who received rFVIIa than among subjects who received placebo, particularly among those who were 65 years of age or older (9.0 % versus 3.8 %, p = 0.003); the rates were especially high among subjects 75 years of age or older (10.8 % versus 4.1 %, p = 0.02). The authors concluded that in a large and comprehensive cohort of persons in placebo-controlled trials of rFVIIa, treatment with high doses of rFVIIa on an off-label basis significantly increased the risk of arterial but not venous thromboembolic events, especially among the elderly.
In an editorial that accompanied the afore-mentioned study, Aledort (2010) stated that "[t]his article uniquely presents data on the off-label use of rFVIIa in a variety of clinical conditions, and it specifically addresses the incidence of thromboembolic events. In patients with bleeding disorders, the challenge is to establish hemostasis without incurring thromboembolic events. Clinical trials are put in place to evaluate the safety and efficacy of new agents. Data and safety monitoring boards, as independent entities, are charged with the responsibility of determining the safety of drugs in a given clinical trial. If the board determines that the data demonstrate that the risk outweighs the benefit, the trial may be halted, but the reasons for stopping the trial may not be widely promulgated. Even if trials are not discontinued, regulators, such as the Food and Drug Administration or the European Medicines Agency, when reviewing a portfolio of information about a drug, may not approve a label indication because of adverse events; however, the data on the adverse events often do not reach the public record. Therefore, patients and health care professionals may have no way to learn about adverse events associated with off-label use".
Furthermore, the editorialist stated that "[t]he authors appropriately warn readers that these data warrant scrutiny when rFVIIa is used on an off-label basis. The thrombotic sequelae reported here are not inconsequential. The risk is particularly notable among older patients. This article should serve as a template for pharmaceutical companies to report all studies involving the use of a given drug, on-label and off-label, so that physicians can fully appreciate the benefit and risks when making therapeutic decisions".
The Canadian Agency for Drugs and Technologies in Health's technology assessment on rFVIIa for the prevention of bleeding unrelated to hemophilia (Murphy et al, 2010) concluded that "no consistent benefit of rFVIIa therapy was detected among studies evaluating the prevention of bleeding in patients undergoing prostatectomy, liver transplantation, or cardiac surgery. The risk of adverse events after the prophylactic use of rFVIIa in surgical patients is unknown. No conclusions can be drawn on the effectiveness or safety of using rFVIIa in the prevention of bleeding in patients who have received supra-therapeutic doses of anticoagulant agents. When used for prevention of bleeding, no specific method is available to monitor the effectiveness of rFVIIa".
Mannucci et al (2010) stated that the main cause of the hemostasis defects and related bleeding complications in patients with acute coronary syndromes (ACS) are the intake of multiple anti-thrombotic drugs, alone or concomitantly with invasive procedures such as coronary angiography and percutaneous coronary intervention (PCI). Anti-thrombotic drugs that impair several phases of hemostasis (platelet function, coagulation, and fibrinolysis) are causing bleeding particularly in elderly patients, in those who are under-weight and with co-morbidities such as renal insufficiency, diabetes, hypertension and malignancy. Identification of patients at high risk of bleeding is the most important preventive strategy, because the choice and dosages of drugs may to some extent be tailored to the degree of risk. Transfusions of blood products, which may become necessary in patients with major bleeding, should be used with caution, because they are associated with adverse cardiovascular events. To reduce the need of transfusion, the hemostatic drugs that decrease blood loss and transfusion requirements in cardiac surgery (anti-fibrinolytic amino acids, desmopressin, and rFVIIa) might be considered. However, the efficacy of these drugs in the control of bleeding complications is not unequivocally established in ACS and there is concern for an increased risk of thrombosis. The authors concluded that evidence-based recommendations for the management of bleeding in patients with ACS are currently lacking, so that prevention through accurate assessment of the individual risk is the most valid strategy.
A Cochrane review found no reliable evidence from randomized controlled clincial trials to support the effectiveness of hemostatic drugs in reducing mortality or disability in patients with traumatic brain injury. Perel et al (2010) evaluated the effects of hemostatic drugs on mortality, disability and thrombotic complications in patients with traumatic brain injury (TBI). These investigators included published and unpublished randomized controlled trials comparing hemostatic drugs (anti-fibrinolytics: aprotinin, tranexamic acid (TXA), aminocaproic acid or rFVIIa) with placebo, no treatment, or other treatment in patients with acute TBI. Two review authors independently examined all electronic records, and extracted the data. They judged that there was clinical heterogeneity between trials so they did not attempt to pool the results of the included trials. The results are reported separately. Two trials were included; one was a post-hoc analysis of 30 TBI patients from a randomized controlled trial of rFVIIa in blunt trauma patients. The risk ratio for mortality at 30 days was 0.64 (95 % CI: 0.25 to 1.63) for rFVIIa compared to placebo. This result should be considered with caution as the subgroup analysis was not pre-specified for the trial. The other trial evaluated the effect of rFVIIa in 97 TBI patients with evidence of intra-cerebral bleeding in a computed tomography (CT) scan. The corresponding risk ratio for mortality at the last follow-up was 1.08 (95 % CI: 0.44 to 2.68). The quality of the reporting of both trials was poor so it was difficult to assess the risk of bias. The authors concluded that there is no reliable evidence from randomized controlled trials to support the effectiveness of hemostatic drugs in reducing mortality or disability in patients with TBI. New randomized controlled trials assessing the effects of hemostatic drugs in TBI patients should be conducted. These trials should be large enough to detect clinically plausible treatment effects.
Human AT III (Thrombate III) is indicated for the treatment of patients with hereditary AT III deficiency in connection with surgical or obstetrical procedures, or when they suffer from thromboembolism.
Rogers (2009) stated that anti-thrombin (AT) functions as a potent natural anticoagulant and serine protease inhibitor that inactivates many enzymes in the coagulation cascade. Antithrombin also possesses anti-inflammatory properties, many of which are mediated by its actions as an anti-coagulant. Hereditary AT deficiency is a rare, under-recognised medical condition that is associated with inadequate endogenous anti-coagulation thought to result from impaired inhibition of serine protease coagulation factors. Inherited as an autosomal dominant trait, congenital AT deficiency typically reduces functional AT levels to 40 to 60 % of normal. As a result, individuals with hereditary AT deficiency have a greater than or equal to 50 % lifetime risk of venous thromboembolism (VTE). Specifically, AT deficiency is associated with a 3- to 7-fold higher risk of VTE compared with other thrombophilias. Thus, maintaining adequate levels of AT during high-risk periods is an important treatment goal. Long-term anti-coagulant thrombo-prophylaxis is not recommended in asymptomatic patients with AT deficiency because of the increased risk of hemorrhage. However, treatment guidelines recommend short-term thromboprophylaxis in high-risk clinical settings, including surgery, trauma, and management of pregnancy, labor, and delivery. The goal of treatment for patients with hereditary AT deficiency is an initial increase in AT activity to greater than or equal to 120 % of normal levels followed by maintenance of AT activity at greater than or equal to 80 % of normal levels. Plasma-derived AT, heparin, fresh frozen plasma, and human recombinant AT are treatment options for individuals with hereditary AT deficiency.
Tiede et al (2008) stated that during surgery and childbirth, patients with hereditary AT deficiency are at high risk for thrombosis, and heparin prophylaxis may not be sufficiently effective. In these patients, exogenous AT may be used in association with heparin. A recombinant human AT has been developed. In a phase III multi-center study, these investigators assessed the safety and effectiveness of prophylactic intravenous administration of AT alfa to hereditary AT deficient patients in high risk situations, including elective surgery, childbirth, or cesarean section. Anti-thrombin alfa was administered prior to and during the high risk period for restoration and maintenance of AT activity at 100 % of normal. Heparin, low-molecular-weight heparin, and/or vitamin K antagonists were used according to standard of care. The primary efficacy endpoint was the incidence of acute deep vein thrombosis (DVT) from baseline up to day 30 post dosing as assessed by independent central review of duplex ultrasonograms and/or venograms. Safety was assessed based on AEs and laboratory evaluations. Five surgical and 9 obstetrical hereditary AT deficiency patients received AT alfa for a mean period of 7 days. No clinically overt DVT occurred. Central review of ultrasonograms identified signs of acute DVT in 2 out of 13 evaluable patients. No AT alfa-related AEs were reported. No patient developed anti-AT alfa antibodies. The authors concluded that these findings suggested that AT alfa is a safe and effective alternative to human plasma-derived AT for treating hereditary AT deficiency patients at high risk for thromboembolic events.
A Cochrane review concluded that AT III can not be recommended for critically ill patients based on the available evidence. Afshari et al (2008) noted that AT III is an anti-coagulant with anti-inflammatory properties but the efficacy and any harmful effects of AT III supplementation in critically ill patients are unknown. These researchers evaluated the benefits and harms of AT III in critically ill patients. They searched the Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library); MEDLINE; EMBASE; Science Citation Index Expanded; International Web of Science; CINAHL; LILACS; and the Chinese Biomedical Literature Database (up to November 2006); and contacted authors and manufacturers in the field. They included all randomized clinical trials, irrespective of blinding or language, that compared AT III with no intervention or placebo in critically ill patients. The primary outcome measure was mortality. These investigators each independently abstracted data and resolved any disagreements by discussion. They presented pooled estimates of the intervention effects on dichotomous outcomes as relative risks (RR) with 95 % CI; performed subgroup analyses to assess risk of bias, the effect of AT III in different populations (sepsis, trauma, obstetric, and pediatric patients), and the effect of AT III in patients with or without the use of concomitant heparin. They assessed the adequacy of the available number of participants and performed a trial sequential analysis to establish the implications for further research. These researchers included 20 randomized trials with a total of 3,458 participants; 13 of these trials had high risk of bias. When they combined all trials, AT III did not statistically significantly reduce overall mortality compared with the control group (RR 0.96, 95 % CI: 0.89 to 1.03; no heterogeneity between trials). A total of 32 subgroup and sensitivity analyses were carried out. Analyses based on risk of bias, different populations, and the role of adjuvant heparin gave insignificant differences. Anti-thrombin III reduced the multi-organ failure score among survivors in an analysis involving very few patients. It increased bleeding events (RR 1.52, 95 % CI: 1.30 to 1.78). The authors concluded that AT III can not be recommended for critically ill patients based on the available evidence. The authors stated that a randomized controlled trial of AT III, without adjuvant heparin, with prespecified inclusion criteria and good bias protection is needed.
Anti-inhibitor coagulant complex, factor eight inhibitor bypassing activity-vapor heated (FEIBA VH), is indicated for the control of spontaneous bleeding episodes or to cover surgical interventions in hemophilia A and hemophilia B patients with inhibitors. In addition, the use of FEIBA VH has been described in a few non-hemophiliacs with acquired inhibitors to factors VIII, XI, and XII.
The development of inhibitory antibodies to factor VIII is a serious complication of hemophilia. Two hemostatic agents with different bypassing mechanisms have been used in the treatment of patients with inhibitors: (i) rFVIIa and (ii) activated prothrombin complex concentrate (APCC). Berntorp (2009) noted that the bypassing agents FEIBA anti-inhibitor coagulant complex and rFVIIa have been established as safe and effective therapies for treating bleeding episodes in hemophilia patients with inhibitors. However, the efficacy of each bypassing agent can vary, and neither agent is universally effective. The reasons for such variability have yet to be confirmed, but may involve patient-specific factors and the mechanisms of action and pharmacokinetic profiles of these 2 agents. Gomperts et al (2008) noted that FEIBA VH and rFVIIa are currently available to circumvent the need for factor FVIII in hemophilia A patients with inhibitors, yet their hemostatic efficacy can be unpredictable. As the results of the FEIBA NovoSeven study illustrated, patients may respond better to one bypassing agent than the other. Furthermore, guidelines from an expert panel reflect that responsiveness to bypassing therapy may change from one bleed to the next in the same patient and even from hour to hour during the course of a single bleeding event. These findings underscored the need to have both bypassing products available to treat bleeding episodes in inhibitor patients, to frequently evaluate the efficacy of hemostasis during the course of a bleeding event, and to switch products early if the response to treatment is unsatisfactory.
A cost-effectiveness analysis found that a rFVIIa regimen appears to be a less expensive treatment option in inhibitor patients with minor-to-moderate bleeds. Joshi et al (2006) compared the cost-effectiveness of 3 treatment regimens using rFVIIa, and APCC (FEIBA vapor heated), for home treatment of minor-to-moderate bleeds in hemophilia patients with inhibitors. The regimens consisting of 1st-, 2nd-, and 3rd-line treatments were: rFVIIa-rFVIIa-rFVIIa; APCC-rFVIIa-rFVIIa; and APCC-APCC-rFVIIa. Patients not responding to 1st-line treatment were administered 2nd-line treatment, and those failing 2nd-line received 3rd-line treatment. Using literature and expert opinion, the model structure and base-case inputs were adapted to the U.S. from a previously published analysis. The percentage of evaluable bleeds controlled with rFVIIa and APCC were obtained from published literature. Drug costs (2005 US$) based on average wholesale price were included in the base-case model. Uni-variate and probabilistic sensitivity analyses (2nd-order Monte Carlo simulation) were conducted by varying the efficacy, re-bleeding rates, patient weight, and dosing to ascertain robustness of the model. In the base-case analysis, the average cost per resolved bleed using rFVIIa as 1st-, 2nd-, and 3rd-line treatment was $28,076. Using APCC as 1st-line and rFVIIa as 2nd- and 3rd-line treatment resulted in an average cost per resolved bleed of $30,883, whereas the regimen using APCC as 1st- and 2nd-line, and rFVIIa as 3rd-line treatment was the most expensive, with an average cost per resolved bleed of $32,150. Cost offsets occurred for the rFVIIa-only regimen through avoidance of 2nd and 3rd lines of treatment. In probabilistic sensitivity analyses, the rFVIIa-only strategy was the least expensive strategy more than 68 % of the time. The authors concluded that the management of minor-to-moderate bleeds extends beyond the initial line of treatment, and should include the economic impact of re-bleeding and failures over multiple lines of treatment. In the majority of cases, the rFVIIa-only regimen appears to be a less expensive treatment option in inhibitor patients with minor-to-moderate bleeds over 3 lines of treatment.
Steen Carlsson et al (2008) compare cost and outcome of APCC and rFVIIa in the treatment of joint bleeds. The analyses were based on the FENOC (FEIBA NovoSeven Comparative Study) cross-over study where 48 patients used APCC and rFVIIa to treat 2 joint bleeds. Incremental cost-effectiveness ratios were calculated for 3 outcome measures and the variation in cost was analyzed using 2 alternative regression methods. Results were subjected to sensitivity analyses. Key determinants of cost were prescribed dose, body weight and treatment in addition to protocol. The cost of APCC was on average lower than rFVIIa. At all but one time-point, patients rated slightly higher (but not statistically significantly) percentages of treatment efficacy and stopping of the bleed by APCC. The reported reduction in pain from start of treatment up to 48 hours varied considerably among individuals. The different relative prices in the U.S., Turkey and Sweden mattered, but did not reverse the main results. The authors concluded that the cost per episode was significantly lower for APCC. The large individual-level variation in reduction of pain supports decisions that consider the individual patient's experience and that accept trade-offs between cost and reduction in pain rather than focusing on cost only.
In an unified Bayesian meta-regression model, Treur et al (2009) analyzed the published efficacy of rFVIIa and/or APCC as on-demand treatments for joint bleeds in hemophilia patients with inhibitors. A systematic search was carried out to identify studies reporting on dosage and efficacy of rFVIIa and APCC in the treatment of joint bleeds in the target patient population. Data were abstracted and included in the model and adjusted for potential sources of heterogeneity. Pooled efficacy levels for typical rFVIIa and APCC regimens were estimated. A total fo 17 studies, collectively reporting on more than 2,000 joint bleeds, were included. Medication type combined with dosage was the only significant explanatory parameter. The model predicts that a typical regimen of 90 microg kg(-1) rFVII repeated every 3 hours if needed results in cumulative joint bleed resolution of 66 %, 88 % and 95 % after 12, 24 and 36 hours, respectively. In comparison, a typical regimen of 75 IU kg(-1) APCC repeated every 12 hours if needed results in cumulative joint bleed resolution of 39 %, 62 % and 76 %, respectively. These differences were statistically significant and were also robust in sensitivity analyses. This analysis suggested that a typical rFVIIa regimen will resolve joint bleeds more effectively than a typical APCC regimen after 12, 24 and 36 hours.
Congenital factor XIII deficiency is rare and affects 1 out of every 3 to 5 million people in the United States. It is associated with a tendency for severe bleeding, a risk for spontaneous abortion, and a high rate of spontaneous intra-cranial hemorrhage. The clinical severity of this deficiency requires regular prophylaxis from the time of diagnosis. Accordingly, replacement material can be infused at intervals as long as every 20 to 30 days. Three types of factor XIII-containing product are available: fresh-frozen plasma (FFP; preferably virus-inactivated), cryoprecipitate, and a pasteurized plasma concentrate (Fibrogammin-P, Dade-Behring). The pasteurized concentrate and virus-inactivated FFP, when available, are preferred to cryoprecipitate (Lovejoy et al, 2006; Mannucci et al, 2010).
On February 17, 2011, the FDA approved Corifact (factor XIII concentrate [Human]), derived from the pooled plasma of healthy donors, via orphan-drug designation for the prevention of bleeding in people with congenital factor XIII deficiency. The FDA approved Corifact based on results of a clinical study of 14 people, including children, with congenital factor XIII deficiency. The most common side effects observed were hypersensitivity reactions (allergy, rash, pruritus, and erythema), chills, fever, arthralgia, headache, elevated thrombin-antithrombin levels, and an increase in hepatic enzymes. It potentially can cause adverse events from abnormal clotting if doses higher than the labeled dose are given to patients.
The U.S. Food and Drug Administration approved recombinant coagulation factor XIII A-subunit (Tretten, Novo Nordisk) for use in the routine prevention of bleeding in adults and children who have a congenital Factor XIII A-subunit deficiency. Tretten received orphan-drug designation for this use by the FDA because it is intended for treatment of a rare disease or condition. Congenital Factor XIII deficiency is a rare genetic disorder. Factor XIII is composed of two subunits, A and B. Factor XIII deficiency is usually caused by a deficiency of the A-subunit.
Tretten is a recombinant analogue of the human Factor XIII A-subunit that is produced in yeast cells and then further purified. It is a sterile freeze-dried-powder to be reconstituted with diluent and injected intravenously. Tretten can be administered by a physician or be self-administered. The effectiveness of coagulation factor XIII A-subunit was studied in 77 patients with congenital Factor XIII A-subunit deficiency. The study found that coagulation factor XIII A-subunit was effective in preventing bleeding in 90 percent of the patients when given monthly. Among the side effects reported in this study were headache, pain in the extremities and pain at injection site. No individual in the trial developed abnormal clots.
The FDA-approved labeling for Tretten states that the dose for routine prophylaxis for bleeding in patients with congenital factor XIII (FXIII) A-subunit deficiency is 35 international units (IU) per kilogram body weight once monthly to achieve a target trough level of FXIII activity at or above 10% using a validated assay. The labeling states that dose adjustment should be considered if adequate coverage is not achieved with the recommended 35 IU/kg dose.
Logan et al (2011) stated that rFVIIa is increasingly used for off-label indications. In a retrospective database analysis, these investigators estimated patterns of off-label rFVIIa use in U.S. hospitals. Data were extracted from the Premier Perspectives database (Premier, Charlotte, NC), which contains discharge records from a sample of academic and non-academic U.S. hospitals. A total of 12,644 hospitalizations for patients who received rFVIIa during a hospital stay were analyzed. Hospital diagnoses and patient dispositions from 1 January 2000 to 31 December 2008 were reviewed. Statistical weights for each hospital were used to provide national estimates of rFVIIa use. From 2000 to 2008, off-label use of rFVIIa in hospitals increased more than 140-fold, such that in 2008, 97 % (95 % CI: 96 % to 98 %) of 18,311 in-hospital uses were off-label. In contrast, in-hospital use for hemophilia increased less than 4-fold and accounted for 2.7 % (CI: 1.9 % to 3.5 %) of use in 2008. Adult and pediatric cardiovascular surgery (29 % CI: 21 % to 33 %), body and brain trauma (29 % CI: 19 % to 38 %), and intracranial hemorrhage (11 % CI: 7.7 % to 14 %) were the most common indications for rFVIIa use. Across all indications, in-hospital mortality was 27 % (CI: 19 % to 34 %) and 43 % (CI: 26 % to 59 %) of patients were discharged to home. The authors concluded that off-label use of rFVIIa in the hospital setting far exceeds use for approved indications. These patterns raise concern about the application of rFVIIa to conditions for which strong supporting evidence is lacking.
Yank et al (2011) evaluated the benefits and harms of rFVIIa use for 5 off-label, in-hospital indications: (i) cardiac surgery, (ii) ICH, (iii) liver transplantation, (iv) prostatectomy, and (v) trauma. A total of 10 databases (including PubMed, EMBASE, and the Cochrane Library) queried from inception through December 2010. Articles published in English were analyzed. Two reviewers independently screened titles and abstracts to identify clinical use of rFVIIa for the selected indications and identified all randomized, controlled trials (RCTs) and observational studies for full-text review. Two reviewers independently assessed study characteristics and rated study quality and indication-wide strength of evidence. A total of 16 RCTs, 26 comparative observational studies, and 22 non-comparative observational studies met inclusion criteria. Identified comparators were limited to placebo (RCTs) or usual care (observational studies). For ICH, mortality was not improved with rFVIIa use across a range of doses. Arterial thrombo-embolism was increased with medium-dose rFVIIa use (risk difference [RD], 0.03 [95 % CI: 0.01 to 0.06]) and high-dose rFVIIa use (RD, 0.06 [CI: 0.01 to 0.11]). For adult cardiac surgery, there was no mortality difference, but there was an increased risk for thrombo-embolism (RD, 0.05 [CI: 0.01 to 0.10]) with rFVIIa. For body trauma, there were no differences in mortality or thrombo-embolism, but there was a reduced risk for the acute respiratory distress syndrome (RD, -0.05 [CI: -0.02 to -0.08]). Mortality was higher in observational studies than in RCTs. The authors concluded that limited available evidence for 5 off-label indications suggested no mortality reduction with rFVIIa use. For some indications, it increases thrombo-embolism.
In an editorial that accompanied the afore-mentioned studies, Avorn and Kesselheim (2011) stated that overall, Yank and co-workers found no evidence that rFVIIa reduced mortality for any off-label use; however, it did increase the risk for thrombo-embolism. Their findings are compatible with other recent studies. The editorialists noted that "[a]llowing physician autonomy to choose medications is appealing, but not when it results in unhelpful, dangerous, and costly decisions. With such compelling data in place about the runaway use, uselessness, and risk for this expensive treatment, what can be done to reduce it? First, if evidence should emerge that the manufacturer played a role in building a market for the unauthorized and increasingly implausible prescribing of its product, both civil and criminal responses will probably be brought to bear, as has occurred for many other instances of corporate-sponsored drug misuse. Second, rFVIIa is used in hospitals, which should be providing organizational oversight to protect patients, as well as the institutions’ own pharmacy budgets. In hospitals where such use continues, existing quality assurance, patient safety, and risk-management groups will surely want to look hard at these practices. Although off-label prescribing by physicians is not illegal, physicians who persist in such use in the face of clear evidence of inutility and harm could be subject to civil action by the affected patients or their heirs".
Koncar et al (2011) examined the influence of rFVIIa on the treatment of intractable peri-operative bleeding in vascular surgery when conventional hemostatic measures are inadequate. There were 2 groups of patients: the NovoSeven group (group N), 10 patients with ruptured abdominal aortic aneurysms (RAAAs) and 14 patients operated on due to thoraco-abdominal aortic aneurysms (TAAAs); the control group (group C), 14 patients with RAAAs and 17 patients with TAAAs. All patients suffered intractable hemorrhage refractory to conventional hemostatic measures, while patients from group N were additionally treated with rFVIIa. Post-operative blood loss was significantly lower in group N treated with rFVII (p < 0.0001). Post-operative administration of packed red blood cells, fresh frozen plasma, and platelets was lower in patients from group N, (p < 0.0001). Successful hemorrhage arrest was reported in 21 patients (87.5 %) treated with rFVIIa, and in 9 patients (29.03 %) in group C (p < 0.001). Thirty-day mortality in these 2 groups significantly differed. The mortality rate was 12.5 % (3 patients) in group N and 80.65 % (25 patients) in group C (p < 0.0001). The authors concluded that these findings suggested that rFVIIa may play a role in controlling the intractable peri-operative and post-operative bleeding in surgical patients undergoing a repair of RAAAs and TAAAs. They stated that prospective randomized trials are necessary to further confirm the efficacy and cost-effectiveness of rFVIIa in these patients.
Witmer et al (2011) described the off-label use of rFVIIa in tertiary care pediatric hospitals across the United States and to assess thrombotic events. A retrospective multi-center cohort study using the Pediatric Health Information System administrative database was performed. Children 18 years of age or younger who received rFVIIa between 2000 and 2007 were included. A label admission was defined as an admission with an International Classification of Diseases diagnostic code for hemophilia or factor VII deficiency; admissions without these codes were classified as off-label. There were 4,942 rFVIIa admissions, representing 3,764 individual subjects; 74 % (3,655) of the admissions were off-label. There was a 10-fold increase in the annual rate of off-label admissions from 2000 to 2007 (from 2 to 20.8 per 10,000 hospital admissions, p < 0.001). The mortality rate in the off-label group was 34 % (1,258/3,655). Thrombotic events occurred in 10.9 % (399/3,655) of the off-label admissions. The authors concluded that off-label use of rFVIIa in hospitalized children is increasing rapidly despite the absence of adequate clinical trials demonstrating safety and efficacy. Thrombotic events are common and mortality is high among patients receiving off-label rFVIIa. They stated that further studies are warranted to determine whether these adverse events are attributable to rFVIIa.
In a Cochrane review, Lin et al (2011) evaluated the effectiveness of rFVIIa when used therapeutically to control active bleeding, or prophylactically to prevent (excessive) bleeding in patients without hemophilia. These investigators searched the Cochrane Injuries Group Specialized Register, CENTRAL, MEDLINE, EMBASE and other specialized databases up to 25 February 2009. Randomized controlled trials comparing rFVIIa with placebo, or one dose of rFVIIa with another, in any patient population (except hemophilia) were included in this study. Outcomes were mortality, blood loss or control of bleeding, red cell transfusion requirements, number of patients transfused and thrombo-embolic adverse events. Two authors independently assessed potentially relevant studies for inclusion, extracted data and examined risk of bias. They considered prophylactic and therapeutic rFVIIa studies separately. A total of 25 RCTs were included: 24 were placebo-controlled double-blind RCTs and 1 compared different doses of rFVIIa. Fourteen trials involving 1,137 participants examined the prophylactic use of rFVIIa; 713 received rFVIIa. There was no evidence of mortality benefit (RR 1.06; 95 % CI: 0.50 to 2.24). There was decreased blood loss (WMD -272 ml; 95 % CI: -399 to -146) and decreased red cell transfusion requirements (WMD -243 ml; 95 % CI: -393 to -92) with rFVIIa treatment; however these values were likely over-estimated due to the inability to incorporate data from trials showing no difference of rFVIIa treatment compared to placebo. There was a trend in favor of rFVIIa in the number of participants transfused (RR 0.91; 95 % CI: 0.82 to 1.02). But there was a trend against rFVIIa with respect to thrombo-embolic adverse events (RR 1.32; 95 % CI: 0.84 to 2.06). Eleven trials involving 2,366 participants examined the therapeutic use of rFVIIa; 1,507 received rFVIIa. There were no outcomes where any observed advantage, or disadvantage, of rFVIIa over placebo could not have been observed by chance alone. There was a trend in favor of rFVIIa for reducing mortality (RR 0.89; 95 % CI: 0.77 to 1.03). However, there was a trend against rFVIIa for increased thrombo-embolic adverse events (RR 1.21; 9 5% CI: 0.93 to 1.58). The authors concluded that the effectiveness of rFVIIa as a more general hemostatic drug, either prophylactically or therapeutically, remains unproven. They stated that the use of rFVIIa outside its current licensed indications should be restricted to clinical trials.
Wilate is a human plasma-derived, sterile, purified, double virus inactivated von Willebrand factor/coagulation factor VIII Complex (human). It is indicated for the treatment of spontaneous and/or trauma-induced bleeding episodes in individuals with severe von Willebrand disease (VWD) or individuals with mild or moderate VWD when there is failure, contraindication or intolerance to desmopressin. It is not indicated for individuals with hemophilia A; and for the prophylaxis of spontaneous bleeding episodes, or the prevention of excessive bleeding during and after surgery in individuals with VWD. The most common adverse reactions to treatment with Wilate in patients with VWD have been urticaria and dizziness. The most serious adverse reactions to treatment with Wilate in patients with VWD have been hypersensitivity reactions.
An UpToDate review on “Factor VIII and factor IX inhibitors in patients with hemophilia” (Hoots and Shapiro, 2012a) does not mention the use of von Willebrand factor/coagulation factor VIII complex (Wilate). Moreover, an UpToDate review on “Treatment of hemophilia” (Hoots and Shapiro, 2012b) states that “Humate P, one of these products, contains both factor VIII and von Willebrand factor, and is mainly used for the treatment of von Willebrand disease. It is not a preferred agent for the treatment of patients with hemophilia A who do not have inhibitors”.
Franchini et al (2013) summarized the current knowledge on treatment strategies for hemophilia B, focusing on recombinant FIX (rFIX) products either clinically used or in development. There is only 1 rFIX product that is licensed to treat hemophilia B patients. From the analysis of the literature data presented in this review, the authors concluded that this rFIX product has demonstrated an excellent safety profile and excellent clinical effectiveness for halting and preventing bleeds in hemophilia B patients. While prophylaxis has emerged as the best therapeutic strategy for such patients because of its ability to prevent hemophilic arthropathy, and to improve patients' quality of life, the pharmacokinetically tailored dosing of rFIX is another key point when planning hemophilia B treatment, as it allows optimization of the factor concentrate usage.
Windyga et al (2014) stated that BAX326 is a rFIX manufactured without the addition of any materials of human or animal origin, and with 2 viral inactivation steps (solvent/detergent treatment and 15 nm nano-filtration). The aim of this prospective trial was to investigate the pharmacokinetics, hemostatic efficacy and safety of BAX326 in previously treated patients aged 12 to 65 years with severe or moderately severe hemophilia B. BAX326 was safe and well-tolerated in all 73 treated subjects; adverse events considered related to treatment (2.7 % incidence, all non-serious) were transient and mild, and no hypersensitivity reactions, inhibitor formation or thrombotic events were observed. Pharmacokinetic (PK) equivalence (n = 28) between BAX326 and a licensed rFIX was confirmed in terms of the ratio of geometric mean AUC0-72 h per dose. Twice-weekly prophylaxis [mean duration 6.2 (+/- 0.7) months; 1.8 (+/- 0.1) infusions per week, 49.5 (+/- 4.8) IU kg-1 per infusion] was effective in preventing bleeding episodes, with a significantly lower (79 %, p < 0.001) annualized bleed rate (4.2) compared to an on-demand treatment in a historical control group (20.0); 24 of 56 subjects on prophylaxis (43 %) did not bleed throughout the study observation period. Of 249 total acute bleeds, 211 (84.7 %) were controlled with 1 to 2 infusions of BAX326. Hemostatic efficacy at resolution of bleed was rated excellent or good in 96.0 % of all treated bleeding episodes. The authors concluded that these findings indicated that BAX326 is safe and effectives in treating bleeds and routine prophylaxis in patients aged 12 years and older with hemophilia B.
On June 27, 2013, the FDA approved Rixubis [Coagulation Factor IX (Recombinant)] for use in people with hemophilia B who are 16 years of age and older. Rixubis is indicated for the control and prevention of bleeding episodes, peri-operative (period extending from the time of hospitalization for surgery to the time of discharge) management, and routine use to prevent or reduce the frequency of bleeding episodes (prophylaxis). Rixubis is a purified protein produced by recombinant DNA technology. It does not contain human or animal proteins. It is supplied in single-use vials of freeze-dried powder and is administered by intravenous injection after reconstitution with sterile water for injection. When used for the routine prevention of bleeding episodes, it is administered twice-weekly. The effectiveness of Rixubis was evaluated in a multi-center study in which a total of 73 male patients between 12 and 65 years of age received Rixubis for routine prophylaxis or as needed in response to symptoms of bleeding (on-demand). Overall, patients in the prophylaxis study had a 75 % lower annual bleeding rate when compared to patients who have historically received on-demand treatment. An additional study in a pediatric population is currently ongoing. Although serious side effects including anaphylaxis can occur, the most common side effects observed in patients in clinical studies were dysgeusia, pain in an extremity, and atypical blood test results.
An UpToDate review on “Treatment of hemophilia” (Hoots, 2013) states that “Recombinant human factor IX has been genetically engineered by insertion of the human factor IX gene into a Chinese hamster ovary cell line. It has been proven to be safe and effective in the treatment of patients with previously treated and previously untreated hemophilia B, with a half-life of 16 to 17 hours. The product has no added albumin, giving it a theoretical advantage over plasma-derived concentrates. The volume of distribution of recombinant factor IX is larger than that for plasma-derived factor IX, with a more pronounced increase in infants and children. Available products include BeneFIX and Rixubis, which was licensed by the US Food and Drug Administration in 2013”.
The U.S. Food and Drug Administration approved Alprolix (Biogen Idec, Cambridge, MA), Coagulation Factor IX (Recombinant), Fc Fusion Protein, for use in adults and children who have hemophilia B to help control and prevent bleeding episodes, manage bleeding during surgical procedures, and prevent or reduce the frequency of bleeding episodes (prophylaxis). Alprolix is designed to require less frequent injections when used to prevent or reduce the frequency of bleeding. Alprolix consists of the Factor IX molecule linked to a protein fragment, Fc, to make the product last longer in circulation. Alprolix received orphan-drug designation for this use by the FDA because it is intended for treatment of a rare disease or condition.
The safety and efficacy of Alprolix were evaluated in a multi-center clinical trial that compared each of two prophylactic treatment regimens to on-demand treatment. Powell, et al. (2013) conducted a phase 3, nonrandomized, open-label study of the safety, efficacy, and pharmacokinetics of Alprolix for prophylaxis, treatment of bleeding, and perioperative hemostasis in 123 previously treated male patients. All participants were 12 years of age or older and had severe hemophilia B (endogenous factor IX level of ≤2 IU per deciliter, or ≤2% of normal levels). The study included four treatment groups: group 1 received weekly dose-adjusted prophylaxis (50 IU of Alprolix per kilogram of body weight to start), group 2 received interval-adjusted prophylaxis (100 IU per kilogram every 10 days to start), group 3 received treatment as needed for bleeding episodes (20 to 100 IU per kilogram), and group 4 received treatment in the perioperative period. A subgroup of group 1 underwent comparative sequential pharmacokinetic assessments of recombinant factor IX and Alprolix. The primary efficacy end point was the annualized bleeding rate, and safety end points included the development of inhibitors and adverse events. As compared with recombinant factor IX, Alprolix exhibited a prolonged terminal half-life (82.1 hours) (P<0.001). The median annualized bleeding rates in groups 1, 2, and 3 were 3.0, 1.4, and 17.7, respectively. In group 2, 53.8% of participants had dosing intervals of 14 days or more during the last 3 months of the study. In groups 1, 2 and 3, 90.4% of bleeding episodes resolved after one injection. Hemostasis was rated as excellent or good during all major surgeries. No inhibitors were detected in any participants receiving Alprolix; in groups 1, 2, and 3, 73.9% of participants had at least one adverse event, and serious adverse events occurred in 10.9% of participants. These events were mostly consistent with those expected in the general population of patients with hemophilia. The authors concluded that prophylactic Alprolix, administered every 1 to 2 weeks, resulted in low annualized bleeding rates in patients with hemophilia B.
One IU of Alprilox per kg body weight increases the circulating level of Factor IX by 1%. The recommended dose for routine prophylaxis is 50 IU/kg once weekly or 100 IU/kg once every 10 days, with dosing regimen adjusted based upon individual response. For control of minor and moderate bleeding episodes (for example, uncomplicated hemarthoses, superficial muscle bleeding (except iliopsoas) without neurovascular compromise, superficial soft tissue bleeding, bleeding of mucous membranes) requires a circulating factor IX level of 30 to 60 IU/dL or 30 to 60 percent of normal). For control of major bleeding (for example, iliopsoas and deep muscle bleeding with neurovascular injury, or substantial blood loss; pharyngea, retropharyngeal, retroperitoneal or CNS bleeding) requires a circulating factor IX level of 80 to 100 IU/dL or 80 to 100 percent of normal. For minor surgery (including uncomplicated dental extraction), the required circulating Factor IX level is 50 to 80 IU/dL or 50 to 80% normal. For major surgery, the required circulating Factor IX level is 60 to 100 IU/dL or 60 to 100 percent of normal.
Patients receiving chronic anti-coagulation therapy with warfarin and other vitamin K antagonist (VKA) anti-coagulants to prevent blood clotting in conditions such as atrial fibrillation or the presence of an artificial heart valve sometimes develop acute bleeding. Hickey et al (2013) determined adverse event frequency after urgent reversal with frozen plasma versus the prothrombin complex concentrate (PCC) -- Octaplex. This natural before-after retrospective cohort study in 2 tertiary care emergency departments compared anti-coagulation reversal with frozen plasma (September 2006 to August 2008) and with Octaplex (September 2008 to August 2010), without other system changes. These researchers included adult patients on warfarin with an international normalized ratio (INR) of greater than or equal to 1.5 who received frozen plasma or Octaplex. The primary outcome was serious adverse events (death, ischemic stroke, myocardial infarction, heart failure, venous thromboembolism, or peripheral arterial thromboembolism) within 7 days. Secondary outcomes included time to INR reversal, hospital length of stay, and red blood cells transfused within 48 hours. They included 149 patients receiving frozen plasma and 165 receiving Octaplex. The incidence of serious adverse events for the frozen plasma group was 19.5 % compared with 9.7 % for the Octaplex group (p = 0.014; relative risk, 2.0; 95 % CI: 1.1 to 3.5). This remained significant after adjustment for baseline history and reason for treatment (p = 0.038; adjusted relative risk, 1.85; 95 % CI: 1.03 to 3.3) in multi-variable regression analysis. Median INR reversal was 11.8 hours with frozen plasma and 5.7 hours with Octaplex (p < 0.0001). Mean red cell transfusion was 3.2 with frozen plasma and 1.4 with Octaplex (p < 0.0001). The authors concluded that Octaplex for urgent reversal of warfarin resulted in faster reversal and lower red cell transfusion requirement with fewer adverse events than frozen plasma.
Hanke et al (2013) noted that the rapid reversal of the effects of VKA is often required in cases of emergency surgery and life-threatening bleeding, or during bleeding associated with high morbidity and mortality such as intra-cranial hemorrhage. Increasingly, 4-factor PCCs (4F-PCC) containing high and well-balanced concentrations of vitamin K-dependent coagulation factors are recommended for emergency oral anti-coagulation reversal. Both the safety and effectiveness of such products are currently in focus, and their administration is now expanding into the critical care setting for the treatment of life-threatening bleeding and coagulopathy resulting either peri-operatively or in cases of acute trauma. After 15 years of clinical use, findings of a pharmaco-vigilance report (February 1996 to March 2012) relating to the 4F-PCC Beriplex P/N (CSL Behring, Marburg, Germany) were analyzed and were presented here. Furthermore, a review of the literature with regard to the safety and effectiveness of 4F-PCCs was performed. Since receiving marketing authorization (February 21, 1996), approximately 647,250 standard applications of Beriplex P/N have taken place. During this time, 21 thromboembolic events judged to be possibly related to Beriplex P/N administration have been reported, while no incidences of viral transmission or heparin-induced thrombocytopenia were documented. The low risk of thromboembolic events reported during the observation period (1 in approximately 31,000) is in line with the incidence observed with other 4F-PCCs. The authors concluded that in general, 4F-PCCs have proven to be well-tolerated and highly effective in the rapid reversal of VKA.
Sarode et al (2013) performed a prospective clinical trial to compare non-activated 4F-PCC with plasma for urgent VKA reversal. In this phase IIIb, multi-center, open-label, non-inferiority trial, non-surgical patients were randomized to 4F-PCC (containing coagulation factors II, VII, IX, and X and proteins C and S) or plasma. Primary analyses examined whether 4F-PCC was non-inferior to plasma for the co-primary end-points of 24-hour hemostatic efficacy from start of infusion and INR correction (less than or equal to 1.3) at 0.5 hour after end of infusion. The intention-to-treat efficacy population comprised 202 patients (4F-PCC, n = 98; plasma, n = 104). Median (range) baseline INR was 3.90 (1.8 to 20.0) for the 4F-PCC group and 3.60 (1.9 to 38.9) for the plasma group. Effective hemostasis was achieved in 72.4 % of patients receiving 4F-PCC versus 65.4 % receiving plasma, demonstrating non-inferiority (difference, 7.1 % [95 % CI: -5.8 to 19.9]). Rapid INR reduction was achieved in 62.2 % of patients receiving 4F-PCC versus 9.6 % receiving plasma, demonstrating 4F-PCC superiority (difference, 52.6 % [95 % CI: 39.4 to 65.9]). Assessed coagulation factors were higher in the 4F-PCC group than in the plasma group from 0.5 to 3 hours after infusion start (p < 0.02). The safety profile (adverse events, serious adverse events, thromboembolic events, and deaths) was similar between groups; 66 of 103 (4F-PCC group) and 71 of 109 (plasma group) patients experienced greater than or equal to 1 adverse event. The authors concluded that 4F-PCC is an effective alternative to plasma for urgent reversal of VKA therapy in major bleeding events, as demonstrated by clinical assessments of bleeding and laboratory measurements of INR and factor levels.
On April 29, 2013, the FDA approved Kcentra (prothrombin complex concentrate, human) for the urgent reversal of acquired coagulation factor deficiency induced by VKA (e.g., warfarin) therapy in adult patients with acute major bleeding. Kcentra is not indicated for urgent reversal of VKA anti-coagulation in patients without acute major bleeding. The FDA approval of Kcentra was based on a study of 216 patients who had been receiving VKA anti-coagulation and who had acute major bleeding along with a clotting test value indicative of anti-coagulant use. Kcentra was demonstrated to be similar to plasma in terms of the ability to stop acute major bleeding. Kcentra is made from the pooled plasma of healthy donors. It is processed in a way to minimize the risk of transmitting viral and other diseases.
Ferreira and DeLosSantos (2013) stated that PCC is an inactivated concentrate of factors II, IX, and X, with variable amounts of factor VII. Guidelines recommend the use of PCC in the setting of life-threatening bleeds, but little is known on the most effective dosing strategies and how the presenting international normalized ratio affects response to therapy. These investigators high-lighted available data on monitoring techniques, address shortcomings of currently available data, the reversal of life-threatening and critical bleeds with PCC, and how this product compares to other therapeutic options used in critically ill patients. Prothrombin complex concentrate has been identified as a potential therapy for critically bleeding patients, but patient-specific factors, product availability, and current data should weigh the decision to use it. Most data exist regarding patients experiencing VKA-induced bleeding, more specifically, those with intra-cranial hemorrhage. Prothrombin complex concentrate has also been studied in trauma-induced hemorrhage; however, it remains controversial, as its potential benefits have the abilities to become flaws in this setting. The authors concluded that health care professionals must remain aware of the differences in products and interpret how 3- versus 4-factor products may affect patients, and interpret literature accordingly. The clinician must be cognizant of how to progress when treating a bleeding patient, propose a supported dosing scheme, and address the need for appropriate factor VII supplementation. At this point, PCC cannot be recommended for first-line therapy in patients with traumatic hemorrhage, and should be reserved for refractory bleeding until more data are available.
The National Institute for Health and Clinical Excellence’s clinical guideline on “Acute upper gastrointestinal bleeding: Management” (NICE, 2012) recommended PCC to patients who are taking warfarin and actively bleeding.
Dewhirst (2013) performed a short-cut review to establish whether rFVIIa improves survival and functional outcome in acute spontaneous intracranial hemorrhage. A total of 92 papers were found using the reported searches, of which 2 presented the best evidence to answer the clinical question. The author, date and country of publication, patient group studied, study type, relevant outcomes, results and study weaknesses of these best papers were tabulated. The authors concluded that current evidence does not support the use of rFVIIa in acute spontaneous intracranial hemorrhage.
Awad and Cocchio (2013) stated that PCC products are emerging as alternative strategies for reversing anticoagulant pharmacotherapy. Factor eight inhibitor bypassing activity (FEIBA, or anti-inhibitor coagulant complex) is an activated PCC (aPCC). Although FEIBA is approved by the FDA to control spontaneous bleeding episodes and to prevent bleeding with surgical interventions in hemophilia A and hemophilia B patients with inhibitors to factor VIII, recent data have suggested that the product may be used off-label as an anticoagulant-reversal agent. These researchers evaluated the safety and effectiveness of aPCC products in reversing anticoagulant pharmacotherapy. They searched online databases for English-language publications that discussed this topic. The EMBASE, MEDLINE, and International Pharmaceutical Abstracts databases were used. These researchers evaluated all articles published in the English language identified from the data sources. They included studies conducted in human subjects and in in-vitro and in-vivo models in this review. Current published evidence suggested that the use of an aPCC, compared with fresh-frozen plasma, is associated with a significantly faster correction of supra-therapeutic INRs secondary to warfarin therapy. Conflicting evidence exists regarding the ability of aPCCs to reverse the prolonged bleeding times caused by the anticoagulant agents including dabigatran etexilate (Pradaxa), rivaroxaban (Xarelto), apixaban (Eliquis), and fondaparinux (Arixtra). The authors concluded that the theoretical risks of thrombosis associated with PCC products must be carefully considered before they are administered to patients who require coagulation therapy. The use of aPCCs to reverse the anticoagulant effects of warfarin, dabigatran, or rivaroxaban should be limited because of the lack of safety and effectiveness data in humans. Moreover, the safety of aPCCs in off-label indications has not been adequately assessed.
Stewart and Pettit (2013) summarized their experiences with FEIBA for the reversal of warfarin-related bleeding in a community hospital. A protocol was put in place in March of 2011, which outlined the use of FEIBA for the emergent reversal of warfarin-related coagulopathy. A fixed low-dose was given based on INR. For an INR less than 5.0, 500 U of FEIBA was administered’ for an INR greater than or equal to 5.0, 1,000 U of FEIBA was given. Intravenous vitamin K was given concurrently regardless of INR. A total of 16 patients were treated with FEIBA per the protocol. Average patient age was 73 years. Intracranial hemorrhage was the most common indication for reversal. Mean pre-treatment INR was 3.56 (1.3 to 6.8); mean post-treatment INR was 1.16 (1.01 to 1.32). Two of the patients required a second 500-U dose, per the protocol, for an INR that had not yet normalized. Bleeding appeared clinically controlled in 93 % of cases; 87 % of patients survived to discharge. There were no signs or symptoms of thrombosis in any of the cases. The authors concluded that emergent reversal of warfarin utilizing a fixed low-dose of FEIBA appears to be effective, consistent, and safe. Moreover, they stated that further comparator studies with other reversal agents are needed.
Cabral and colleagues (2013) evaluated the safety and effectiveness of a PCC-based protocol in patients with warfarin-associated ICH, sub-dural hematoma (SDH), or sub-arachnoid hemorrhage (SAH). This was a retrospective case-series review of patients treated with an institution-approved warfarin reversal protocol. Patients with ICH and known warfarin use with an INR greater than 1.4 received FFP, vitamin K (phytonadione), and weight-based, 3-factor PCC (Profilnine(®) SD) dose based on the initial INR. Demographic and clinical information, the degree of and time to INR normalization, and adverse events were recorded. The 30 study patients included 19 with primary ICH, 7 with SDH, and 4 with SAH. The mean age was 72.8 (± 11) years, including 11 (37 %) patients greater than or equal to 80 years old. The median presenting INR was 2.3 (IQR 2 to 3.3) and post-treatment INR was 1.4 (IQR 1.3 to 1.5, Z score 6.4, p < 0.001). Median time from PCC administration to the first follow-up INR was 95 (IQR 50 to 140) mins. No patient's INR increased by more than 0.3 over 72 hrs. Nine patients (30 %) underwent neurosurgical procedures after PCC administration and no procedure-related bleeding complication was noted. Adverse events included 3 instances of early hematoma expansion, 1 ischemic stroke in a patient with endocarditis on post-PCC day 1, 1 pulmonary embolism 5 weeks after PCC treatment, and 1 coronary in-stent thrombosis 60 days after PCC treatment. Six patients died prior to hospital discharge of anticipated complications of their initial event, and none from identifiable thrombotic complications of PCC. The authors concluded that a 3-factor PCC preparation (Profilnine(®) SD), administered with FFP and vitamin K to patients with acute warfarin-associated intracranial bleeding is a reasonable approach to urgent warfarin reversal. Moreover, they stated that randomized, prospective trials are needed to verify the safety and clinical effectiveness of PCC administration in this population.
Song et al (2013) reported their initial experience with the PCC FEIBA for the rescue treatment of coagulopathy and life-threatening bleeding after cardiac surgery. A total of 25 patients who underwent cardiac surgery with coagulopathy and life-threatening bleeding refractory to conventional treatment received FEIBA as rescue therapy at the authors’ institution. This cohort represented approximately 2 % of patients undergoing cardiac surgery in the authors’ university-based practice during the study. The patients were at high risk for post-operative coagulopathy with nearly all patients having at least 2 risk factors for this. Aortic root replacement (Bentall or valve-sparing procedure) and heart transplant with or without left ventricular assist device explant were the most common procedures. The mean FEIBA dose was 2,154 units. The need for fresh frozen plasma and platelet transfusion decreased significantly after FEIBA administration (p = 0.0001 and p < 0.0001). The mean INR decreased from 1.58 to 1.13 (p < 0.0001). Clinical outcomes were excellent. No patient returned to the operating room for re-exploration. There was no hospital mortality and all patients were discharged home. One patient who had a central line and trans-venous pacemaker developed an upper extremity DVT. The authors concluded that their initial experience with FEIBA administration for the rescue treatment of post-operative coagulopathy and life-threatening bleeding has been favorable. Moreover, they stated that further studies are indicated to confirm its safety and effectiveness; and determine specific clinical indications for its use in patients undergoing cardiac surgery.
The U.S. Food and Drug Administration (FDA) has approved Eloctate [Antihemophilic Factor (Recombinant), Fc Fusion Protein] for the control and prevention of bleeding episodes, perioperative (surgical) management and routine prophylaxis in adults and children with hemophilia A (Biogen Idec, 2014). Eloctate reduces the frequency of bleeding episodes with prophylactic infusions every three to five days, offering people with hemophilia A the potential to extend the interval between prophylactic infusions. Eloctate was developed by fusing B-domain deleted factor VIII to the Fc portion of immunoglobulin G subclass 1 (IgG1) which enables Eloctate to prolong the time the therapy remains in the body.
The recommended starting prophylactic regimen for Eloctate is 50 IU/kg every four days. Based on clinical response, the regimen may be adjusted in the range of 25 to 65 IU/kg and every three to five days (Biogen Idec., 2014).
In clinical trials, Eloctate was effective for both routine prophylaxis and to treat acute bleeding episodes with a favorable safety and tolerability profile (Biogen Idec, 2014). The approval of Eloctate is based on results from the global, Phase 3 A-LONG clinical study, as well as interim pharmacokinetic and safety data from the Phase 3 Kids A-LONG study.
The A-LONG study was an open-label, multi-center study that examined the efficacy, safety and pharmacokinetics of Eloctate in 165 previously treated males 12 years of age and older with severe hemophilia A (Biogen Idec, 2014). Results showed that adults and adolescents with severe hemophilia A achieved a statistically significant reduction of bleeding episodes in both of the study’s prophylaxis arms, relative to the on-demand treatment arm. In addition, 98 percent of bleeding episodes were controlled with one or two Elocttate infusions.
The study evaluated individualized and weekly prophylaxis to reduce or prevent bleeding episodes, and on-demand dosing to treat bleeding episodes (Biogen Idec, 2014). In the individualized arm, each study participant started on a twice-weekly dosing regimen. Participants’ pharmacokinetic parameters were used to guide adjustments to dosing interval (every three to five days), and dose (25 to 65 IU/kg) to target a minimum factor VIII level of 1 to 3 IU/dL or higher as needed to maintain control of breakthrough bleeding episodes. In the study, the dose in the weekly prophylaxis arm was 65 IU/ kg/week. The overall median annualized bleeding rates (ABR), or projected number of bleeding episodes per year, reported in the study were 1.6 for the individualized prophylaxis arm, 3.6 for the weekly prophylaxis arm and 33.6 for the on-demand arm.
No participants in the A-LONG study developed inhibitors to Eloctate (Biogen Idec, 2014). One participant had a transient, positive neutralizing antibody test result, which was not confirmed upon repeat testing. There were no reports of serious vascular clots or serious allergic reactions. Across the routine prophylaxis and on-demand therapy arms, adverse reactions were reported in 5.5 percent of participants. Adverse reactions included arthralgia, malaise, upper abdominal pain, lower abdominal pain, angiopathy, bradycardia, chest pain, cough, dizziness, dysgeusia, cold and heat intolerance, headache, hypertension, joint swelling, myalgia, procedural hypotension and rash. Each event occurred in two or fewer study participants. Two participants were withdrawn from the study due to adverse reactions: one participant due to rash and one due to arthralgia.
The pediatric indication for Eloctate is supported by interim safety and pharmacokinetic results in 38 boys ages two to 11 years old from the Phase 3 Kids A-LONG study (Biogen Idec, 2014). These data showed that Eloctate was generally well-tolerated and no inhibitors were detected. The relative increase in half-life seen with Eloctate was consistent with findings in adults and adolescents. In comparison with adolescents and adults, children two to five years old have a shorter half-life and higher clearance of hemophilic factors (adjusted for body weight); therefore, a higher dose or more frequent dosing may be needed in this age group. In April 2014, Biogen Idec and Swedish Orphan Biovitrum (Sobi) reported positive top-line results from the completed Kids A-LONG study, which confirmed and expanded upon the interim data. Common adverse reactions (incidence of greater than or equal to 1 percent) reported in the A-LONG study were arthralgia and malaise.